Measuring red blood cell shape in the human retina

Abstract

The free diameter of a red blood cell generally exceeds the lumen diameter of capillaries in the central nervous system, requiring significant cellular deformation. However, the deformations undertaken are not well established under natural conditions due to the difficulty in observing corpuscular flow in vivo. Here we describe a novel, to the best of our knowledge, method to noninvasively study the shape of red blood cells as they traverse the narrow capillary networks of the living human retina, using high-speed adaptive optics. One hundred and twenty-three capillary vessels were analyzed in three healthy subjects. For each capillary, image data were motion-compensated and then averaged ov..